Abstract
The circadian clock responds to light signals and therefore participates in the plant’s daily response to light. The phase response curve (PRC) is typically used in the study of chronobiology to detect the effect of various environmental cues on a given circadian rhythm. In this chapter we describe protocols on measuring the setting of the light pulses at different times of a day, the measurement of circadian rhythm, and the calculation of phase shift in response to light pulses. The promoter:luciferase reporter was used to provide fine rhythmic traces and the subsequent circadian parameters of mathematical analysis. A classical PRC assay to light pulses is the key experimental basis for determining the signal components of resetting the circadian clock.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Greenham K, McClung CR (2015) Integrating circadian dynamics with physiological processes in plants. Nat Rev Genet 16:598–610
Millar AJ (2016) The intracellular dynamics of circadian clocks reach for the light of ecology and evolution. Annu Rev Plant Biol 67:595–618
Johnson CH, J.A, E., and Foster, R. (2003) Entrainment of circadian programs. Chronobiol Int 20:741–774
Aschoff J (1960) Exogenous and endogenous components in circadian rhythms. Cold Spring Harb Symp Quant Biol 25:11–28
Pittendrigh CS, Minis DH (1964) The entrainment of circadian oscillations by light and their role as photoperiodic clocks. Am Nat 98:261–322
Devlin PF, Kay SA (2000) Cryptochromes are required for phytochrome signaling to the circadian clock but not for rhythmicity. Plant Cell 12:2499–2510
Covington MF, Panda S, Liu XL, Strayer CA, Wagner DR, Kay SA (2001) ELF3 modulates resetting of the circadian clock in Arabidopsis. Plant Cell 13:1305–1316
Somers DE, Devlin P, Kay SA (1998) Phytochromes and cryptochromes in the entrainment of the Arabidopsis circadian clock. Science 282:1488–1490
Feher B, Kozma-Bognar L, Kevei E, Hajdu A, Binkert M, Davis SJ, Schafer E, Ulm R, Nagy F (2011) Functional interaction of the circadian clock and UV RESISTANCE LOCUS 8-controlled UV-B signaling pathways in Arabidopsis thaliana. Plant J 67:37–48
Devlin PF, Kay SA (2001) Circadian photoperception. Annu Rev Physiol 63:677–694
Aschoff J (1999) Masking and parametric effects of high-frequency light-dark cycles. Jpn J Physiol 49:11–18
Crosthwaite SK, Loros JJ, Dunlap JC (1995) Light-induced resetting of a circadian clock is mediated by a rapid increase in frequency transcript. Cell 81:1003–1012
Johnson CH (1990). An atlas of phase response curves for circadian and circatidal rhythms, (Nashville: Department of Biology, Vanderbilt University)
Johnson CH (1999) Forty years of PRCs--what have we learned? Chronobiol Int 16:711–743
Pittendrigh CS (1960) Circadian rhythms and the circadian organization of living systems. Cold Spring Harb Symp Quant Biol 25:159–184
Xu X, Xie Q, McClung CR (2010) Robust circadian rhythms of gene expression in Brassica rapa tissue culture. Plant Physiol 153:841–850
Salomé PA, McClung CR (2005) PSEUDO-RESPONSE REGULATOR 7 and 9 are partially redundant genes essential for the temperature responsiveness of the Arabidopsis circadian clock. Plant Cell 17:791–803
Salomé PA, McClung CR (2005) What makes the Arabidopsis clock tick on time?: A review on entrainment. Plant Cell Environ 28:21–38
Haydon MJ, Mielczarek O, Robertson FC, Hubbard KE, Webb AA (2013) Photosynthetic entrainment of the Arabidopsis thaliana circadian clock. Nature 502:689–692
Gutiérrez RA, Stokes TL, Thum K, Xu X, Obertello M, Katari MS, Tanurdzic M, Dean A, Nero DC, McClung CR et al (2008) Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1. Proc Natl Acad Sci U S A 105:4939–4944
Michael TP, Salomé PA, McClung CR (2003) Two Arabidopsis circadian oscillators can be distinguished by differential temperature sensitivity. Proc Natl Acad Sci U S A 100:6878–6883
Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China to X.X. (U1904202) and Q.X. (31670285).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Xu, X., Yuan, L., Xie, Q. (2022). Circadian Rhythm: Phase Response Curve and Light Entrainment. In: Staiger, D., Davis, S., Davis, A.M. (eds) Plant Circadian Networks. Methods in Molecular Biology, vol 2398. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1912-4_1
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1912-4_1
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1911-7
Online ISBN: 978-1-0716-1912-4
eBook Packages: Springer Protocols