Abstract
The molecular characterization of cancer could have significant clinical benefits, including early diagnosis, making treatment decisions, and monitoring therapeutic response. In this regard, noninvasive assessment of expression/activity of specific molecules in tumors could be vital in managing cancer. Optical probes have demonstrated promise in the molecular imaging of cancer. Here, we have described a method to noninvasively assess the activity of matrix metalloproteinases (MMPs) in human prostate tumors in mice. We used an activatable probe MMPSense™ 750 FAST (MMPSense750) with fluorescent properties in the near-infrared (NIR) range with peak excitation at ~749 nm and peak emission ~775 nm. These optical properties offer the advantage of a higher depth of detection. This probe has shown immense potential in imaging MMPs activity in deeper tissue with high target-specific signal and low background autofluorescence. Therefore, this probe could be valuable in assessing MMPs activity in primary tumors and metastasis.
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Acknowledgments
We acknowledge the support provided by DOD awards # W81XWH-15-1-0188; # W81XWH-19-1-0427 (to GD).
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Kim, S., Deep, G. (2022). Optical Imaging of Matrix Metalloproteinases Activity in Prostate Tumors in Mice. In: Deep, G. (eds) Cancer Biomarkers. Methods in Molecular Biology, vol 2413. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1896-7_2
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DOI: https://doi.org/10.1007/978-1-0716-1896-7_2
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