Abstract
The copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, well-known as “click” reaction, is widely used in organic synthesis, medicinal chemistry, and polymer science for the conjugation of molecular entities of all sizes. In this protocol, B-cell epitope J8, derived from group A Streptococcus (GAS) M protein, and universal T-helper epitope PADRE were conjugated to poly(methyl acrylate) (PMA) to form a self-assembled nanoparticle vaccine candidate (PMA-P-J8). The vaccine construct was orally administered to mice in a single dose of 30 μg, resulting in the production of a high number of serum (IgG) and salivary (IgA) antibodies.
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Faruck, M.O., Skwarczynski, M., Toth, I. (2022). Polymer–Peptide Conjugate Vaccine for Oral Immunization. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 2412. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1892-9_3
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DOI: https://doi.org/10.1007/978-1-0716-1892-9_3
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