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Cationic Nanostructures as Adjuvants for Vaccines

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Vaccine Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2412))

Abstract

Spherical or discoidal lipid polymer nanostructures bearing cationic charges successfully adsorb a variety of oppositely charged antigens (Ag) such as proteins, peptides, nucleic acids, or oligonucleotides. This report provides instructions for the preparation and physical characterization of four different cationic nanostructures able to combine and deliver antigens to the immune system: (1) dioctadecyl dimethylammonium bromide (DODAB) bilayer fragments (DODAB BF); (2) polystyrene sulfate (PSS) nanoparticles (NPs) covered with one cationic dioctadecyl dimethylammonium bromide bilayer (DODAB) named (PSS/DODAB); (3) cationic NPs of biocompatible polymer poly(methyl methacrylate) (PMMA) prepared by emulsion polymerization of the methyl methacrylate (MMA) monomer in the presence of DODAB BF (PMMA/DODAB NPs); (4) antigen NPs (NPs) where the cationic polymer poly(diallyl dimethyl ammonium chloride) (PDDA) directly combined at nontoxic and low dose with the antigen (Ag); when the oppositely charged model antigen is ovalbumin (OVA), NPs are named PDDA/OVA. These nanostructures provide adequate microenvironments for carrying and delivering antigens to the antigen-presenting cells of the immune system.

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Acknowledgments

This work was supported by grant 2019/17685-2, São Paulo Research Foundation (FAPESP) and 302758/2019-4, Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico (CNPq). Y.P.-B is the recipient of a PhD fellowship from CNPq.

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Authors and Affiliations

  1. Biocolloids Laboratory, Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil

    Ana Maria Carmona-Ribeiro, Beatriz Ideriha Mathiazzi & Yunys Pérez-Betancourt

Authors
  1. Ana Maria Carmona-Ribeiro
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  2. Beatriz Ideriha Mathiazzi
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  3. Yunys Pérez-Betancourt
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Correspondence to Ana Maria Carmona-Ribeiro .

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Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, PA, USA

    Sunil Thomas

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Carmona-Ribeiro, A.M., Mathiazzi, B.I., Pérez-Betancourt, Y. (2022). Cationic Nanostructures as Adjuvants for Vaccines. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 2412. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1892-9_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1892-9_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1891-2

  • Online ISBN: 978-1-0716-1892-9

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