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Construction of Genomic and Sub-Genomic Dengue Infectious Replicons

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Dengue Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2409))

Abstract

Dengue replicons are powerful tools used in studying virus biology as well as in high-throughput screening of drug candidates. Replicon constructs are developed as genomic (consists of all the viral protein genes) or sub-genomic (consists of only nonstructural protein genes) and are used to study various aspects of the virus life cycle such as genome replication and virus assembly. In addition, a replicon usually includes a reporter gene used in monitoring virus replication. In this chapter, we provide methods to develop both genomic and sub-genomic dengue replicons with a luciferase reporter and describe different assays to utilize these systems.

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Acknowledgments

The research was supported by National Institute of Health grant AI073755. We thank Dr. Rushika Perera, Dr. Shishir Poudyal, and Sheryl Kelly for helpful discussion and assistance.

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Authors and Affiliations

  1. Department of Biological Sciences, Purdue University, West Lafayette, IN, USA

    Thu M. Cao & Richard J. Kuhn

  2. Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, IN, USA

    Thu M. Cao & Richard J. Kuhn

Authors
  1. Thu M. Cao
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  2. Richard J. Kuhn
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Corresponding author

Correspondence to Richard J. Kuhn .

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Editors and Affiliations

  1. Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Ronaldo Mohana-Borges

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Cao, T.M., Kuhn, R.J. (2022). Construction of Genomic and Sub-Genomic Dengue Infectious Replicons. In: Mohana-Borges, R. (eds) Dengue Virus. Methods in Molecular Biology, vol 2409. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1879-0_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1879-0_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1878-3

  • Online ISBN: 978-1-0716-1879-0

  • eBook Packages: Springer Protocols

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