Abstract
Trans-kingdom RNA interference (RNAi) has been reported in several plant-fungal pathosystems. Our recent works have demonstrated natural RNAi transmission from cotton plants into Verticillium dahliae, a soil-borne phytopathogenic fungus that infects host roots and proliferates in vascular tissues, and successful application of trans-kingdom RNAi in cotton plants to confer Verticillium wilt disease resistance. Here, we provide a detailed protocol of cotton infection with V. dahliae, fungal hyphae recovery from infected cotton stems, and transmitted small RNA detection developed from our previous studies for trans-kingdom RNAi assays.
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Acknowledgments
This work was supported by a grant from the National Natural Science Foundation of China (31730078 to H.-S.G. and 31700131 to Y.J.).
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Zhang, T., Wang, F., Guo, HS., Jin, Y. (2022). Trans-Kingdom RNA Silencing in Plant-Fungal Disease Control. In: Mysore, K.S., Senthil-Kumar, M. (eds) Plant Gene Silencing. Methods in Molecular Biology, vol 2408. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1875-2_16
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DOI: https://doi.org/10.1007/978-1-0716-1875-2_16
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