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Membrane and Nuclear Yeast Two-Hybrid Systems

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Plant Virology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2400))

Abstract

The yeast two-hybrid (Y2H) system is an effective means of detecting protein–protein interactions through the activation of reporter gene expression. This system has also displayed several useful applications, including rapidly revealing unexpected interactors of known proteins, detecting regions that take part in protein–protein interactions, and characterizing the orchestration of protein interactions in metabolic pathways. The Y2H system has been widely utilized in the study of plant virology to investigate interactions between viral proteins and host, vector, or viral proteins. This technology has been successful in enhancing the understanding of viral structure and assembly, replication, viral gene expression and regulation, viral movement, pathogenicity, and the network of viral proteins. Here, we provide the methods to uncover novel interactors of viral proteins via screening cDNA libraries of plant host and insect vectors, using nuclear and membrane Y2H systems, respectively.

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Acknowledgments

This work was supported by grants from the National Key R&D Program of China (2017YFD0200900) and the National Natural Science Foundation of China (31772124 and 31972239) to Q.C.

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Authors and Affiliations

  1. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, People’s Republic of China

    Qian Chen & Taiyun Wei

Authors
  1. Qian Chen
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  2. Taiyun Wei
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Corresponding author

Correspondence to Taiyun Wei .

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Editors and Affiliations

  1. London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada

    Aiming Wang

  2. State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China

    Yi Li

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Chen, Q., Wei, T. (2022). Membrane and Nuclear Yeast Two-Hybrid Systems. In: Wang, A., Li, Y. (eds) Plant Virology . Methods in Molecular Biology, vol 2400. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1835-6_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1835-6_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1834-9

  • Online ISBN: 978-1-0716-1835-6

  • eBook Packages: Springer Protocols

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