Abstract
In planta gene expression analysis and GFP-based confocal microscopy are two powerful techniques that may be coupled to assess the extent and dynamics of plant colonization by a fungal pathogen. Here we describe methods to prepare common bean plants for inoculation with a highly virulent strain of Fusarium oxysporum f. sp. phaseoli, quantify the extent of colonization by RT-qPCR, and visualize the colonized tissues by confocal microscopy.
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Acknowledgments
We are indebted to Michael R. Thon for critical reading of the manuscript and many suggestions. This work was supported by Ministerio de Ciencia e Innovación grant PID2019-110605RB-100 (Spain).
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Castillo, V.Cd., Benito, E.P., Díaz-Mínguez, J.M. (2022). In Planta Gene Expression Analysis and Colonization of Fusarium oxysporum. In: Coleman, J. (eds) Fusarium wilt. Methods in Molecular Biology, vol 2391. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1795-3_12
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DOI: https://doi.org/10.1007/978-1-0716-1795-3_12
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