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Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon Resonance

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Mycobacterium ulcerans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2387))

Abstract

Many pathogenic bacteria utilize glycan-based interactions to bind to host cells. Glycan array analysis and surface plasmon resonance are glycobioanalytical techniques that have been used to investigate the glycointeractions of a range of pathogens. The analysis of the glycointeractome, particularly the binding of host glycans by Mycobacteria, has been limited. In this chapter, we outline methodologies that have been successfully implemented for studying Mycobacterium ulcerans glycointeractions.

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References

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Acknowledgments

M.P.J. is supported by a National Health and Medical Research Council (NHMRC) Program Grant (1071659) and an NHMRC Principal research fellowship (APP1138466). G.P. is supported by a grant from the Medicor Foundation.

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Author notes

  1. Christopher J. Day and Jessica Poole contributed equally with all other contributors.

Authors and Affiliations

  1. Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia

    Christopher J. Day, Jessica Poole & Michael P. Jennings

  2. Swiss Tropical and Public Health Institute, Basel, Switzerland

    Gerd Pluschke

  3. University of Basel, Basel, Switzerland

    Gerd Pluschke

Authors
  1. Christopher J. Day
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  2. Jessica Poole
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  3. Gerd Pluschke
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  4. Michael P. Jennings
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Corresponding author

Correspondence to Michael P. Jennings .

Editor information

Editors and Affiliations

  1. Swiss Tropical and Public Health Institute, Basel, Switzerland

    Gerd Pluschke

  2. Stanford School of Medicine Department of Pathology, Stanford University, Stanford, CA, USA

    Katharina Röltgen

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Day, C.J., Poole, J., Pluschke, G., Jennings, M.P. (2022). Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon Resonance. In: Pluschke, G., Röltgen, K. (eds) Mycobacterium ulcerans. Methods in Molecular Biology, vol 2387. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1779-3_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1779-3_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1778-6

  • Online ISBN: 978-1-0716-1779-3

  • eBook Packages: Springer Protocols

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