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Improvement of Transfection with PepFects Using Organic and Inorganic Materials

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Cell Penetrating Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2383))

Abstract

Cell-penetrating peptides (CPPs) are a promising non-viral vector for gene and drug delivery. CPPs exhibit high cell transfection, and are biocompatible. They can be also conjugated with organic and inorganic nanomaterials, such as magnetic nanoparticles (MNPs), graphene oxide (GO), metal-organic frameworks (MOFs), and chitosan. Nanomaterials offered a high specific surface area and provided relatively straightforward methods to be modified with biomolecules including CPPs and oligonucleotides (ONs). Novel nanomaterials conjugates with CPP/ONs complexes are therefore of interest for cell transfection with high efficiency. In this chapter, we described a summary of the non-viral vectors consisting of CPPs and nanomaterials. The book chapter also included a protocol to generate hybrid biomaterials consisting of CPPs and nanoparticles (NPs) for the delivery of oligonucleotides. The conjugation of NPs with CPPs serves as an effective platform for gene therapy with high cell transfection efficiency. The protocol is simple, offers high cell transfection compared to the CPPs-ONs complexes, and can be used for further improvements using external stimuli.

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Abbreviations

CPPs:

Cell-penetrating peptides

GO:

Graphene oxide

HSA:

Human serum albumin

MNPs:

Magnetic nanoparticles

MOFs:

Metal-organic frameworks

mPEG:

Methoxy polyethylene glycol

MRI:

Magnetic resonance imaging

NIR:

Near-infrared

NP:

Nanoparticle

ON:

Oligonucleotide

PEI:

Polyethyleneimine

PFs:

PepFects

PL-PEG:

Phospholipid-based amphiphilic polymer

R8:

Octa-arginine

rGO:

Reduced graphene oxide

SAH:

Subarachnoid hemorrhage

SMAR1:

Scaffold matrix attachment region binding protein 1

TP10:

Transportan 10

ZIFs:

Zeolitic imidazolate frameworks

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Acknowledgments

This work was supported by The Swedish Research Council, and Swedish Cancer Foundation, Sweden. H.N. Abdelhamid thanks the Ministry of Higher Education and Scientific Research (MHESR) and Institutional Review Board (IRB) of the Faculty of Science at Assiut University, Egypt for the support.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden

    Moataz Dowaidar & Ülo Langel

  2. Advanced Multifunctional Materials Laboratory, Department of Chemistry, Faculty of Science, Assiut University, Assiut, Egypt

    Hani Nasser Abdelhamid

  3. Institute of Technology, University of Tartu, Tartu, Estonia

    Ülo Langel

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  1. Moataz Dowaidar
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  2. Hani Nasser Abdelhamid
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  3. Ülo Langel
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  1. Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden

    Ülo Langel

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Dowaidar, M., Abdelhamid, H.N., Langel, Ü. (2022). Improvement of Transfection with PepFects Using Organic and Inorganic Materials. In: Langel, Ü. (eds) Cell Penetrating Peptides. Methods in Molecular Biology, vol 2383. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1752-6_35

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  • DOI: https://doi.org/10.1007/978-1-0716-1752-6_35

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