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Cell-Penetrating Peptides

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Cell Penetrating Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2383))

Abstract

In this introductory chapter, we first define cell-penetrating peptides (CPPs), give short overview of CPP history and discuss several aspects of CPP classification. Next section is devoted to the mechanism of CPP penetration into the cells, where direct and endocytic internalization of CPP is explained. Kinetics of internalization is discussed more extensively, since this topic is not discussed in other chapters of this book. At the end of this section some features of the thermodynamics of CPP interaction with the membrane is also presented. Finally, we present different cargoes that can be transferred into the cells by CPPs and briefly discuss the effect of cargo on the rate and efficiency of penetration into the cells.

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Authors and Affiliations

  1. Medical Faculty, Institute of Biochemistry and Molecular Genetics, University of Ljubljana, Ljubljana, Slovenia

    Matjaž Zorko

  2. Department of Biochemistry and Biophysics, University of Stockholm, Stockholm, Sweden

    Ülo Langel

  3. Institute of Technology, University of Tartu, Tartu, Estonia

    Ülo Langel

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  1. Matjaž Zorko
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  1. Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden

    Ülo Langel

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Zorko, M., Langel, Ü. (2022). Cell-Penetrating Peptides. In: Langel, Ü. (eds) Cell Penetrating Peptides. Methods in Molecular Biology, vol 2383. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1752-6_1

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