Abstract
CXCR5+ memory CD4+ T cells in human blood represent a circulating compartment of T follicular helper (Tfh) cells (termed circulating Tfh: cTfh cells). cTfh cells are composed of subsets that differ in phenotype and functions, and include resting and activated cell populations. Analysis of cTfh cell subsets have proven to be valuable to assess the ongoing global Tfh cell responses in vivo in distinct medical conditions including autoimmune diseases, infectious diseases, and after vaccination. Herein we will describe a detailed protocol to analyze cTfh subsets in human blood samples and to assess their helper capacity in culture with B cells.
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Acknowledgments
This work was supported by grant from the US National Institutes of Health (AI153673), and Japan Agency for Medical Research and Development.
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Iwamoto, Y., Ueno, H. (2022). Circulating T Follicular Helper Subsets in Human Blood. In: Graca, L. (eds) T-Follicular Helper Cells. Methods in Molecular Biology, vol 2380. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1736-6_3
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DOI: https://doi.org/10.1007/978-1-0716-1736-6_3
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