Abstract
Antibodies produced by plasma cells are a major arm of adaptive immunity. Germinal center reactions that include germinal center B cells and follicular T cells are fundamental players for antibody production, particularly antigen specific antibodies. Here we describe multiple methods that we and others have developed to analyze the production of antigen-specific antibodies in mice, with protocols for assessing antibody affinity and antibody isotype. The detection of antigen-specific IgE in serum using a traditional enzyme-linked immunosorbent assay (ELISA) method is often problematic due to much higher amounts of IgG. Thus we provide a specialized protocol for the detection of antigen-specific IgE in serum using ELISA.
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Acknowledgements
We thank Drs. Koji Iijima and Hirohita Kita (Mayo Clinic) for their help with the Ag-specific IgE protocol. We also thank Dr. Carola Vinuesa (Australian National University) and Dr. Stephanie Eisenbarth (Yale University) for helpful suggestions on food allergy induction protocols.
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Xie, M.M., Dent, A.L. (2022). Assessing In Vivo T Cell-Dependent Antigen-Specific Antibody Responses. In: Graca, L. (eds) T-Follicular Helper Cells. Methods in Molecular Biology, vol 2380. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1736-6_14
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DOI: https://doi.org/10.1007/978-1-0716-1736-6_14
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