Skip to main content

Extraction, Characterization, and Identification of Odorous Metabolites from Streptomyces

  • Protocol
  • First Online:
Methods in Actinobacteriology

Abstract

This protocol describes screening of the actinobacterial isolates for the production of odorous metabolites by olfactory analysis, extraction and characterization of the odor compounds in potent Streptomyces spp. by gas chromatography–mass spectrometry, and identification of the gene responsible for the odor production in Streptomyces spp. by PCR.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 299.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Berthelot M, Andre G (1891) Sur l’odeur propre de la terre. Compt Rend 112:598–599

    Google Scholar 

  2. Scholler CE, Gurtler H, Pedersen R, Molin S, Wilkins K (2002) Volatile metabolites from actinomycetes. J Agric Food Chem 50(9):2615–2621

    Article  Google Scholar 

  3. Tung S, Lin T, Tseng I, Lin H (2006) Identification of 2-MIB and geosmin producers in Feng-Shen reservoir in South Taiwan. Water Supply 6(2):55–61

    Article  CAS  Google Scholar 

  4. Effmert U, Kalderás J, Warnke R, Piechulla B (2012) Volatile mediated interactions between bacteria and fungi in the soil. J Chem Ecol 38(6):665–703

    Article  CAS  Google Scholar 

  5. Forbes SL, Perrault KA (2014) Decomposition odor profiling in the air and soil surrounding vertebrate carrion. PLoS One 9(4):e95107

    Article  Google Scholar 

  6. Gerber NN, Lechevalier HA (1965) Geosmin, an earthy-smelling substance isolated from actinomycetes. Appl Microbiol 13(6):935–938

    Article  CAS  Google Scholar 

  7. Gerber NN (1969) A volatile metabolite of actinomycetes, 2-methylisoborneol. J Antibiot 22(10):508–509

    Article  CAS  Google Scholar 

  8. Hayes JB Jr, Laws ER (1991) Handbook of pesticide toxicology, vol 1. Academic Press, San Diego, CA

    Google Scholar 

  9. Cross T (1981) Aquatic actinomycetes: a critical survey of the occurrence, growth and role of actinomycetes in aquatic habitats. J Appl Bacteriol 50:397–423

    Article  CAS  Google Scholar 

  10. Raschke RL, Carroll B, Tebo LB (1975) The relationship between substrate content, water quality, actinomycetes and musty odors in the Broad River Basin. J Appl Ecol 12:535–560

    Article  CAS  Google Scholar 

  11. Niemi RM, Knuth S, Lundström K (1982) Actinomycetes and fungi in surface waters and in potable water. Appl Environ Microbiol 43:378–388

    Article  CAS  Google Scholar 

  12. Kawato M, Shinobu R (1959) A simple technique for the microscopical observation, memoirs of the Osaka University Liberal Arts and Education, p 114

    Google Scholar 

  13. Brattoli M et al (2011) Odor detection methods: olfactometry and chemical sensors. Sensors 11:5290–5322

    Article  CAS  Google Scholar 

  14. Jensen SE, Anders CL, Goatcher LJ, Perley T, Kenefick S, Hrudey SE (1994) Actinomycetes as a factor in odor problems affecting drinking water from the North Saskatchewan river. Water Res 28(6):1393–1401

    Article  CAS  Google Scholar 

  15. Perry DJ, Pasi KJ (1999) Hemostasis and thrombosis protocols, Methods in molecular medicine. Humana Press, Totowa, p 31

    Book  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Dharumadurai, D., Saravanan, K., Saravanan, C., Govindhan, S., Selvanathan, L. (2022). Extraction, Characterization, and Identification of Odorous Metabolites from Streptomyces. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_94

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-1728-1_94

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1727-4

  • Online ISBN: 978-1-0716-1728-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics