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An In Vitro Nematicidal Activity of Actinobacteria: Juvenile Mortality and Egg Hatching Inhibition

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Methods in Actinobacteriology

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Plant-parasitic nematodes are microscopic, obligatory endoparasites with worldwide distribution can cause severe damage to various agricultural crops. The study of in vitro nematicidal activity of actinobacteria reveals that Streptomyces antibioticus strain M7 against nematode inhibition of juvenile mortality and egg hatching in Meloidogyne incognita. Streptomyces antibioticus strain M7 and actinomycins have potential to develop as safe nematicidal agents to control the root-knot nematodes and to improve the crop yield.

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References

  1. Dong LD, Zhang KQ (2006) Microbial control of plant-parasitic nematodes: a five-party interaction. Plant Soil 288:31–45

    Article  CAS  Google Scholar 

  2. Sun MH, Gao L, Shi YX, Li BJ, Liu XZ (2006) Fungi and actinomycetes associated with Meloidogyne sp., eggs andfemales in China and their biocontrol potential. J Invertebr Pathol 93:22–28

    Article  Google Scholar 

  3. Dicklow MB, Acosta N, Zuckerman BM (1993) A novel Streptomyces species for controlling plant-parasitic nematodes. J Chem Ecol 19:159–173

    Article  CAS  Google Scholar 

  4. Saxena G (2004) Biocontrol of nematode-borne disease in vegetablecrops. In: Mukerji KG (ed) Disease management of fruits andvegetables. Klumer, Netherland, pp 397–450

    Chapter  Google Scholar 

  5. Ruanpanun P, Laatsch H, Tangchitsomkid N, Lumyong S (2011) Nematicidal activity of fervenulin isolated from a nematicidal actinomycete, Streptomyces sp. CMU-MH021, on Meloidogyne incognita. World J Microbiol Biotechnol 27:1373–1380

    Article  CAS  Google Scholar 

  6. Caillaud MC, Dubreuil G, Quentin M, Perfus-Barbeoch L, Lecomte P, de AlmeidaEngler J, Abad P, Rosso MN, Favery B (2008) Root-knot nematodesmanipulate plant cell functions during a compatible interaction. J Plant Physiol 165:104–113

    Article  CAS  Google Scholar 

  7. Bird AF, McClure MA (1976) The tylenchid (Nematoda) egg shell: structure,composition and permeability. Parasitology 72:19–28

    Article  Google Scholar 

  8. Wharton D (1980) Nematode eggshells. Parasitology 81:447–463

    Article  CAS  Google Scholar 

  9. Khan A, Williams KL, Nevalainen HKM (2004) Effects of Paecilomyceslilacinus protease and chitinase on the eggshell structures and hatching of Meloidogynejavenica juveniles. Biol Control 31:346–352

    Article  CAS  Google Scholar 

  10. Curtis RH (2008) Plant–nematode interactions: environmental signals detected by the nematode’s chemosensory organs control changes in the surfacecuticle and behavior. Parasite 15:310–316

    Article  CAS  Google Scholar 

  11. Caboni P, Ntalli NG (2014) Botanical nematicides, recent findings, ACS symposium series, vol 1172. ACS Publications, Washington, DC, pp 145–157

    Google Scholar 

  12. Kaur T, Jasrotia S, Ohri P (2016) Evaluation of in vitro and in vivo nematicidal potential of a multifunctional streptomycete, Streptomyces hydrogenans strain DH16 against Meloidogyne incognita. Microbiological Research 192:247–252

    Google Scholar 

  13. Jin N, Lu X, Wang X, Liu Q, Peng D, Jian H (2019) The effect of combined application of Streptomyces rubrogriseus HDZ-9-47 with soil biofumigation on soil microbial and nematode communities. Scientific Reports 9:16886. https://doi.org/10.1038/s41598-019-52941-9

  14. Young TW (1954) An incubation method for collecting migratory endo-parasitic nematodes. Plant Disease Reporter 38:794–795

    Google Scholar 

  15. Mountain WB, Patrick ZA (1959) The peach replant problem in Ontario. VII. The pathogenicity of Pratylenchus penetrans (Cobb, 1917) Filip. & Stek. 1941. Canadian Journal of Botany 37:459–470

    Google Scholar 

  16. Sun MH, Gao L, Shi YX, Li BJ, Liu XZ (2006) Fungi and Actinomycetes associated with Meloidogyne spp. eggs and females in China and their biocontrol potential. J Invert Pathol 93:22–28

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    Vijayakumar Vishnu Raja & Dharumadurai Dhanasekaran

Authors
  1. Vijayakumar Vishnu Raja
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  2. Dharumadurai Dhanasekaran
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Editors and Affiliations

  1. Department of Microbiology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    Dhanasekaran Dharumadurai

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Raja, V.V., Dhanasekaran, D. (2022). An In Vitro Nematicidal Activity of Actinobacteria: Juvenile Mortality and Egg Hatching Inhibition. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_91

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  • DOI: https://doi.org/10.1007/978-1-0716-1728-1_91

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1727-4

  • Online ISBN: 978-1-0716-1728-1

  • eBook Packages: Springer Protocols

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