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Vascular Tissue Engineering pp 61–75Cite as

Microfluidic Coaxial Bioprinting of Hollow, Standalone, and Perfusable Vascular Conduits

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2375))

Abstract

Three-dimensional bioprinting represents promising approach for fabricating standalone and perfusable vascular conduits using biocompatible materials. Here we describe a step-by-step method by using a multichannel coaxial extrusion system (MCCES) and a blend bioink constituting gelatin methacryloyl, sodium alginate, and eight-arm poly(ethylene glycol)-acrylate with a tripentaerythritol core for the fabrication of standalone circumferentially multilayered hollow tubes. This microfluidic bioprinting method allows the fabrication of perfusable vascular conduits with a core lumen, an inner endothelial layer resembling the tunica intima, and an outer smooth muscle cell layer resembling the tunica media of the blood vessel. Biocompatible and perfusable blood vessels with a widely tunable size range in terms of luminal diameters and wall thicknesses can be successfully fabricated using the MCCES.

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Acknowledgments

This work was supported by funding from the National Institutes of Health (R00CA201603, R21EB025270, R21EB026175, R01EB028143, R01HL153857, R21EB030257), National Science Foundation (CBET-EBMS-1936105), the Brigham Research Institute, and the American Fund for Alternatives to Animal Research (AFAAR). Y.S.Z. sits on the Scientific Advisory Board of Allevi, Inc., which however, did not support or bias this work. The other authors declare no competing financial/commercial interest.

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Authors and Affiliations

  1. Division of Engineering in Medicine, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, USA

    Sushila Maharjan, Jacqueline Jialu He, Li Lv, Di Wang & Yu Shrike Zhang

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  1. Sushila Maharjan
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  2. Jacqueline Jialu He
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  3. Li Lv
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  4. Di Wang
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  5. Yu Shrike Zhang
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Corresponding author

Correspondence to Yu Shrike Zhang .

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Editors and Affiliations

  1. Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA

    Feng Zhao

  2. Department of Biomedical Engineering, Columbia University, New York, NY, USA

    Kam W. Leong

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Maharjan, S., He, J.J., Lv, L., Wang, D., Zhang, Y.S. (2022). Microfluidic Coaxial Bioprinting of Hollow, Standalone, and Perfusable Vascular Conduits. In: Zhao, F., Leong, K.W. (eds) Vascular Tissue Engineering. Methods in Molecular Biology, vol 2375. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1708-3_6

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  • DOI: https://doi.org/10.1007/978-1-0716-1708-3_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1707-6

  • Online ISBN: 978-1-0716-1708-3

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