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Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans

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CRISPR-Cas Methods

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Abstract

CRISPR-Cas9 is the current choice for genome editing for its versatility and specificity. The Cas9 endonuclease makes double-strand breaks at the target sites, which are repaired via non-homologous end-joining or homologous recombination. So far, various genome editing methods using CRISPR-Cas have been developed for Caenorhabditis elegans. However, repairing a double-strand break via homologous recombination is a crucial step to modify a genome in an error-free manner. Here, we focus on a procedure on how to prepare repair templates for precise genome editing via homologous recombination in C. elegans, which applies to a variety of CRISPR-Cas methods.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (NSFC No. 31972876) award and Tianjin University grant to H.M.K. The authors thank Dr. Mario Marchisio for proofreading and the comments.

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Authors and Affiliations

  1. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China

    Hyun-Min Kim & Xiaojuan Zhang

Authors
  1. Hyun-Min Kim
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  2. Xiaojuan Zhang
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Correspondence to Hyun-Min Kim .

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Editors and Affiliations

  1. Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

    M. Tofazzal Islam

  2. ICAR-National Rice Research Institute, Cuttack, Odisha, India

    Kutubuddin Ali Molla

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Kim, HM., Zhang, X. (2021). Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans. In: Islam, M.T., Molla, K.A. (eds) CRISPR-Cas Methods. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1657-4_24

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  • DOI: https://doi.org/10.1007/978-1-0716-1657-4_24

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1656-7

  • Online ISBN: 978-1-0716-1657-4

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