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Testing for Thyroid Peroxidase and Antineuronal Antibodies in Depression and Schizophrenia

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Physical Exercise and Natural and Synthetic Products in Health and Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2343))

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Abstract

Dietary interventions and physical exercise may improve some symptoms in mental illnesses such as major depression and schizophrenia. Hashimoto’s thyroiditis is a known risk factor for these conditions and is marked by the presence of circulating antibodies to thyroid peroxidase (TPO) and thyroglobulin (TG). This chapter presents a protocol to determine if patients with major depression or schizophrenia contain high circulating levels of these antibodies relative to healthy controls. We also describe a procedure testing for the presence of other circulating biomarkers related to brain function, including antibodies directly related to neuronal function. This analysis was performed by screening biochip mosaics of frozen tissue sections and transfected HEK293 cells expressing target antigens using patient and control sera. Finally, we describe a correlation analysis of these markers with symptom scores at baseline and after 6 weeks treatment of the patients using antipsychotics or antidepressants as appropriate.

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Acknowledgments

Anke Dudeck, Sieglinde Funke, Bianca Jerzykiewicz, Wolfgang Jordan, Katrin Brenner, and Jeanette Schadow participated in serum sample characterization and collection.

Conflict of Interest: Dr. Teegen and Prof. Stoecker are full-time employees of and hold stock in Euroimmun AG. All other authors report no conflict of interest.

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Authors and Affiliations

  1. Laboratory of Translational Psychiatry, University of Magdeburg, Magdeburg, Germany

    Johann Steiner, Henrik Dobrowolny, Gabriela Meyer-Lotz & Hans-Gert Bernstein

  2. Department of Psychiatry and Psychotherapy, University of Magdeburg, Magdeburg, Germany

    Johann Steiner, Henrik Dobrowolny, Gabriela Meyer-Lotz & Hans-Gert Bernstein

  3. Center for Behavioral Brain Sciences, Magdeburg, Germany

    Johann Steiner

  4. Institute for Experimental Immunology, Affiliated to EUROIMMUN AG, Luebeck, Germany

    Winfried Stoecker & Bianca Teegen

  5. Institute of Clinical Chemistry and Pathobiochemistry, University of Magdeburg, Magdeburg, Germany

    Katrin Borucki

  6. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

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  1. Johann Steiner
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  2. Winfried Stoecker
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Correspondence to Johann Steiner .

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Editors and Affiliations

  1. Charlesworth House, Debden, Essex, UK

    Paul C. Guest

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Steiner, J. et al. (2022). Testing for Thyroid Peroxidase and Antineuronal Antibodies in Depression and Schizophrenia. In: Guest, P.C. (eds) Physical Exercise and Natural and Synthetic Products in Health and Disease. Methods in Molecular Biology, vol 2343. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1558-4_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1558-4_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1557-7

  • Online ISBN: 978-1-0716-1558-4

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