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Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in Staphylococcus aureus

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Staphylococcus aureus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2341))

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Abstract

Oxygen consumption is a fundamental characteristic of staphylococcal physiology reflecting the energy and metabolic state of the bacterial cell. During aerobic growth, oxygen consumption rates (OCR) depend on nutrient availability and vary at different growth stages. The measurement of oxygen consumption rates provides a versatile tool to characterize the impact of various mutations, environmental cues, and antibiotics on bacterial growth and fitness. In this chapter, we describe a MitoXpress® Xtra-based oxygen consumption assay for fast and reliable determination of respiration rates in Staphylococcus aureus. This highly reproducible and simple method requires a minimal set of reagents and allows rapid screening of multiple samples through real-time determination of the OCR with an oxygen-sensing probe and fluorescence plate reader.

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Author information

Authors and Affiliations

  1. Department of Pathology and Microbiology, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, NE, USA

    Logan L. Bulock, Jennifer L. Endres & Marat R. Sadykov

Authors
  1. Logan L. Bulock
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  2. Jennifer L. Endres
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  3. Marat R. Sadykov
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Corresponding author

Correspondence to Marat R. Sadykov .

Editor information

Editors and Affiliations

  1. IFAS, Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA

    Kelly C. Rice

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Bulock, L.L., Endres, J.L., Sadykov, M.R. (2021). Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in Staphylococcus aureus . In: Rice, K.C. (eds) Staphylococcus aureus. Methods in Molecular Biology, vol 2341. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1550-8_11

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  • DOI: https://doi.org/10.1007/978-1-0716-1550-8_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1549-2

  • Online ISBN: 978-1-0716-1550-8

  • eBook Packages: Springer Protocols

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