Abstract
Neuronal communication mostly relies on a constant adjustment of the number and subtype of neurotransmitter receptors and ion channels at the plasma membrane. Although classical fluorescence ensemble approaches have provided valuable insights into cellular and molecular pathways, their inherent limitations (e.g., average behaviors, spatial resolution) have prompted neuroscientists to use and adapt other imaging approaches. Among these, the single nanoparticle tracking offers a remarkable possibility to explore, at the single neurotransmitter receptor and ion channel level, the behavior of these molecular actors in living brain cells. In this chapter, we describe the procedure and experimental steps necessary to perform single nanoparticle tracking of membrane neurotransmitter receptor and ion channel in living brain cells.
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Acknowledgments
This work was supported by the Centre National de la Recherche Scientifique, the Agence Nationale de la Recherche, the Fondation pour la Recherche Médicale, the Human Frontier Science Program, and the Conseil Régional d’Aquitaine. We thank the cell culture facility of the institute, the animal facility of the University of Bordeaux, and the Bordeaux Imaging Center for technical support and lab members for constructive discussions.
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Varela, J., Dupuis, J., Maingret, F., Groc, L. (2021). Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells. In: Lujan, R., Ciruela, F. (eds) Receptor and Ion Channel Detection in the Brain. Neuromethods, vol 169. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1522-5_5
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DOI: https://doi.org/10.1007/978-1-0716-1522-5_5
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