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Methods to Assay the Behavior of Drosophila melanogaster for Toxicity Study

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Environmental Toxicology and Toxicogenomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2326))

Abstract

Drosophila melanogaster, the fruit fly, has been widely used in biological investigation as an invertebrate alternative to mammals for its various advantages compared to other model organisms, which include short life cycle, easy handling, high prolificacy, and great availability of substantial genetic information. The behavior of Drosophila melanogaster is closely related to its growth, which can reflect the physiological conditions of Drosophila. We have optimized simple and robust behavioral assays for determining the larvae survival, adult climbing ability (mobility assay), reproductive behavior, and lifespan of Drosophila. In this chapter, we present the step-by-step detailed method for studying Drosophila behavior.

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Acknowledgments

This project was funded by the National Natural Science Foundation of China (31671284), the Fundamental Research Funds for the Central Universities (JZ2020HGPA0115), and the Youth Science and Technology Talents Support Program (2020) by Anhui Association for Science and Technology.

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Authors and Affiliations

  1. School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, China

    Guiran Xiao

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  1. Guiran Xiao
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Corresponding author

Correspondence to Guiran Xiao .

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Editors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, NC, USA

    Xiaoping Pan

  2. Department of Biology, East Carolina University, Greenville, NC, USA

    Baohong Zhang

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Xiao, G. (2021). Methods to Assay the Behavior of Drosophila melanogaster for Toxicity Study. In: Pan, X., Zhang, B. (eds) Environmental Toxicology and Toxicogenomics. Methods in Molecular Biology, vol 2326. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1514-0_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1514-0_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1513-3

  • Online ISBN: 978-1-0716-1514-0

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