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Flow Cytofluorometric Analysis of Molecular Mechanisms of Premature Red Blood Cell Death

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Environmental Toxicology and Toxicogenomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2326))

Abstract

This chapter describes, in detail, the operational principles and experimental design to analyze the premature death of human red blood cells (RBCs; erythrocytes). Necrosis (i.e., hemolysis), eryptosis, and necroptosis are the three types of cell death thus far known to exist in RBCs, and distinctive markers of each are well established. Here, methods based on flow cytometry are presented in an easily reproducible form. Moreover, manipulation of incubation medium to promote or inhibit certain physiological phenomena, along with a step-by-step approach to examine membrane scrambling, cell volume, surface complexity, calcium activity, oxidative stress, and signal transduction pathways are also discussed.

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Acknowledgements

The authors are grateful to the Deanship of Scientific Research, King Saud University for funding this research project through Vice Deanship of Scientific Research Chairs (DSRVCH).

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Authors and Affiliations

  1. Chair of Medical and Molecular Genetics Research, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia

    Mohammad A. Alfhili

  2. Division of Hematology/Oncology, Department of Internal Medicine, Brody School of Medicine, East Carolina University, Greenville, NC, USA

    Myon Hee Lee

Authors
  1. Mohammad A. Alfhili
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  2. Myon Hee Lee
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Corresponding authors

Correspondence to Mohammad A. Alfhili or Myon Hee Lee .

Editor information

Editors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, NC, USA

    Xiaoping Pan

  2. Department of Biology, East Carolina University, Greenville, NC, USA

    Baohong Zhang

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Alfhili, M.A., Lee, M.H. (2021). Flow Cytofluorometric Analysis of Molecular Mechanisms of Premature Red Blood Cell Death. In: Pan, X., Zhang, B. (eds) Environmental Toxicology and Toxicogenomics. Methods in Molecular Biology, vol 2326. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1514-0_11

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  • DOI: https://doi.org/10.1007/978-1-0716-1514-0_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1513-3

  • Online ISBN: 978-1-0716-1514-0

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