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Differentiation of Midbrain Dopaminergic Neurons from Human iPS Cells

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Experimental Models of Parkinson’s Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2322))

Abstract

Human-induced pluripotent stem (iPS) cells provide a powerful means for analyzing disease mechanisms and drug screening, especially for neurological diseases, considering the difficulty to obtain live pathological tissue. The midbrain dopaminergic neurons of the substantia nigra are mainly affected in Parkinson’s disease, but it is impossible to obtain and analyze viable dopaminergic neurons from live patients. This problem can be overcome by the induction of dopaminergic neurons from human iPS cells. Here, we describe an efficient method for differentiating human iPS cells into midbrain dopaminergic neurons. This protocol holds merit for obtaining a deeper understanding of the disease and for developing novel treatments.

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Acknowledgments

This work was supported by Grants-in-Aid for Scientific Research (20K07873 to K.I., 20K07741 to R.N.) from the Japan Society for the Promotion of Science (JSPS), MEXT-Supported Programs for the Strategic Research Foundation at Private Universities (S1411007), Fostering Physicians in Basic Research for Coping with Advancing Sophistication of Medicine and Medical Care, and the Rare/Intractable Disease Project of Japan (JP17ek0109244 to K.I. and W.A).

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Authors and Affiliations

  1. Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Kei-Ichi Ishikawa & Risa Nonaka

  2. Center for Genomic and Regenerative Medicine, Juntendo University School of Medicine, Tokyo, Japan

    Kei-Ichi Ishikawa, Risa Nonaka & Wado Akamatsu

Authors
  1. Kei-Ichi Ishikawa
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  2. Risa Nonaka
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  3. Wado Akamatsu
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Corresponding author

Correspondence to Kei-Ichi Ishikawa .

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Editors and Affiliations

  1. Department of Research for Parkinson’s Disease, Juntendo University Graduate School of Medicine, Tokyo, Japan

    Yuzuru Imai

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Ishikawa, KI., Nonaka, R., Akamatsu, W. (2021). Differentiation of Midbrain Dopaminergic Neurons from Human iPS Cells. In: Imai, Y. (eds) Experimental Models of Parkinson’s Disease. Methods in Molecular Biology, vol 2322. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1495-2_8

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  • DOI: https://doi.org/10.1007/978-1-0716-1495-2_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1494-5

  • Online ISBN: 978-1-0716-1495-2

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