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Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human-Induced Pluripotent Stem Cells

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Pluripotent Stem-Cell Derived Cardiomyocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2320))

Abstract

Human iPSC-derived cardiomyocytes (hiPSC-CMs) are expected to be used in regenerative therapies and drug discovery for heart failure. hiPSC-CMs are a mixture of mainly ventricular CMs (VCMs) and also of atrial CMs (ACMs) and pacemaker cells. Here we describe a method to enrich VCM and ACM differentiation and to characterize these subtypes by gene expression analysis using qRT-PCR and by electrophysiological properties using the patch-clamp method. The differentiated VCMs and ACMs highly express VCM and ACM marker genes, respectively. Furthermore, both subtypes show specific properties of action potentials.

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Acknowledgments

We thank Peter Karagiannis for proof-reading the manuscript. This work was supported by a grant from Takeda Pharmaceutical Company Limited, a grant from Leducq foundation (18CVD05), JSPS KAKENHI Grants (19K16041 and 17H04176), grants from the Research Center Network for Realization of Regenerative Medicine (JP19bm0104001, JP19bm0204003, and JP19bm0804008), Research on Regulatory Science of Pharmaceuticals and Medical Devices (JP19mk0104117), and Research Project for Practical Applications of Regenerative Medicine (JP19bk0104095) provided by the Japan Agency for Medical Research and Development, and iPS research fund.

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Authors and Affiliations

  1. Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Misato Nakanishi-Koakutsu, Tadashi Takaki & Kenji Miki

  2. Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan

    Misato Nakanishi-Koakutsu, Tadashi Takaki & Kenji Miki

  3. Department of Pancreatic Islet Cell Transplantation, National Center for Global Health and Medicine, Tokyo, Japan

    Tadashi Takaki

  4. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Yoshinori Yoshida

Authors
  1. Misato Nakanishi-Koakutsu
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  2. Tadashi Takaki
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  3. Kenji Miki
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  4. Yoshinori Yoshida
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Corresponding author

Correspondence to Yoshinori Yoshida .

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Editors and Affiliations

  1. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Yoshinori Yoshida

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Nakanishi-Koakutsu, M., Takaki, T., Miki, K., Yoshida, Y. (2021). Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human-Induced Pluripotent Stem Cells. In: Yoshida, Y. (eds) Pluripotent Stem-Cell Derived Cardiomyocytes. Methods in Molecular Biology, vol 2320. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1484-6_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1484-6_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1483-9

  • Online ISBN: 978-1-0716-1484-6

  • eBook Packages: Springer Protocols

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