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Therapeutic Antibodies pp 143–150Cite as

Large-Scale Transient Production in ExpiCHO-S™ with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2313))

Abstract

Large-scale transient expression in Chinese Hamster Ovary (CHO) cells provides a rapid protein production method with a potential start-to-end alignment advantage for biotherapeutics drug discovery. In this chapter, experimental protocols are illustrated for transient expression of therapeutic glycoproteins with improved galactosylation and sialylation in ExpiCHO-S™ system. To reduce the production cost, we also describe a novel procedure for PEI-mediated transfection in ExpiCHO-S™ cells that supports therapeutic protein expression comparable to the level with ExpiFectamine™-based transfection.

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Authors and Affiliations

  1. BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA

    Xiaotian Zhong, Ashley Schwab, Weijun Ma, Caryl L. Meade, Jing Zhou, Aaron M. D’Antona, Will Somers & Laura Lin

Authors
  1. Xiaotian Zhong
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  2. Ashley Schwab
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  3. Weijun Ma
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  4. Caryl L. Meade
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  5. Jing Zhou
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  6. Aaron M. D’Antona
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  7. Will Somers
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  8. Laura Lin
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Corresponding author

Correspondence to Xiaotian Zhong .

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Editors and Affiliations

  1. Department of Neurology, Rigshospitalet, Glostrup, Denmark

    Gunnar Houen

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Zhong, X. et al. (2022). Large-Scale Transient Production in ExpiCHO-S™ with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection . In: Houen, G. (eds) Therapeutic Antibodies. Methods in Molecular Biology, vol 2313. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1450-1_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1450-1_7

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1449-5

  • Online ISBN: 978-1-0716-1450-1

  • eBook Packages: Springer Protocols

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