Abstract
The lack of a convenient, easily maintained, and inexpensive in vitro human neuronal model to study neurodegenerative diseases prompted us to develop a rapid, 1-h differentiated neuronal cell model based on human NT2 cells and C3 transferase. Here, we describe the rapid differentiation of human neuronal NT2 cells, and the differentiation, transduction, and transfection of human SK-N-MC cells and rat PC12 cells to obtain cells with the morphology of differentiated neurons that can express exogenous genes of interest at high level.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Pleasure SJ, Page C, Lee VM (1992) Pure, postmitotic, polarized human neurons derived from NTera 2 cells provide a system for expressing exogenous proteins in terminally differentiated neurons. J Neurosci 12:1802–1815
Katoh M (2002) Regulation of WNT signaling molecules by retinoic acid during neuronal differentiation in NT2 cells: threshold model of WNT action (review). Int J Mol Med 10:683–687
Jain P, Cerone MA, Leblanc AC, Autexier C (2007) Telomerase and neuronal marker status of differentiated NT2 and SK-N-SH human neuronal cells and primary human neurons. J Neurosci Res 85:83–89
Darbinian N, Darbinyan A, Czernik M et al (2008) HIV-1 tat inhibits NGF-induced Egr-1 transcriptional activity and consequent p35 expression in neural cells. J Cell Physiol 216:128–134
Peruzzi F, Gordon J, Darbinian N, Amini S (2002) Tat-induced deregulation of neuronal differentiation and survival by nerve growth factor pathway. J Neurovirol 8(Suppl 2):91–96
Darbinian-Sarkissian N, Czernik M, Peruzzi F et al (2006) Dysregulation of NGF-signaling and Egr-1 expression by tat in neuronal cell culture. J Cell Physiol 208:506–515
Biedler JL et al (1973) Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res 33:2643–2652
Helson L et al (1975) Human neuroblastoma in nude mice. Cancer Res 35:2594–2599
Biedler JL et al (1978) Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res 38:3751–3757
Roomi M, Kalinovsky T, Roomi NW, Niedzwiecki A, Rath M (2013) Inhibition of the SK-N-MC human neuroblastoma cell line in vivo and in vitro by a novel nutrient mixture. Oncol Rep 29(5):1714–1720
Kim KB, Lee S, Kim JH (2020) Neuroprotective effects of urolithin A on H2O2-induced oxidative stress-mediated apoptosis in SK-N-MC cells. Nutr Res Pract 14(1):3–11
Mishra M, Del Valle L, Otte J, et al. (2012) Pur-alpha regulates RhoA developmental expression and downstream signaling. J Cell Physiol 228:65–72
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Darbinian, N. (2021). Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12, and SK-N-MC. In: Amini, S., White, M.K. (eds) Neuronal Cell Culture. Methods in Molecular Biology, vol 2311. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1437-2_3
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1437-2_3
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1436-5
Online ISBN: 978-1-0716-1437-2
eBook Packages: Springer Protocols