Abstract
B cell development is a stepwise process encompassing several B cell precursor stages that can be phenotypically distinguished, and that is taking place in the bone marrow in adults. Interestingly, within the bone marrow B cell precursors coexist with the most differentiated actors of this lineage, the plasma cells. In this chapter, we describe a method to recover cells from the bone marrow and a flow cytometric approach to identify each subpopulation of the B cell lineage that resides within the bone marrow compartment. This protocol focuses on membrane markers to discriminate all the B cell subpopulations in order to preserve cell integrity during experimentation and for further analyses.
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Acknowledgments
The study was supported by an ANR @RAction grant (ANR-14-ACHN-0008), an ANR JCJC grant (ANR-19-CE15-001901), a Fondation ARC grant (PJA20181208173), a grant from IdEx Université de Paris (ANR-18-IDEX-0001) to ME, and an ANR PRC grant (ANR-17-CE14-0019), an INCa grant (PRT-K 2017) and the Association Saint Louis pour la Recherche sur les Leucémies to KB.
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Bonaud, A., Balabanian, K., Espéli, M. (2021). Immunophenotyping of the Medullary B Cell Compartment In Mouse Models. In: Espéli, M., Balabanian, K. (eds) Bone Marrow Environment. Methods in Molecular Biology, vol 2308. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1425-9_8
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DOI: https://doi.org/10.1007/978-1-0716-1425-9_8
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