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Shotgun Bacterial Lipid A Analysis Using Routine MALDI-TOF Mass Spectrometry

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Mass Spectrometry-Based Lipidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2306))

Abstract

Detection of bacterial lipids and particularly the lipid A, the lipid anchor of the lipopolysaccharide, can be very challenging and requires a certain level of expertise. Here, this chapter describes a straightforward and simple method for the analysis of bacterial lipid A. In addition, such approach, lipid fingerprint, has the potential to be applied to other bacteria such as mycobacteria.

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Acknowledgments

This work was supported by the MRC Confidence in Concept Fund and the ISSF Wellcome Trust Grant 105603/Z/14/Z. G.L.-M. is co-inventor of the MALDIxin test for which a patent has been filed by Imperial Innovations WO2018158573.

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Authors and Affiliations

  1. MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, UK

    Gérald Larrouy-Maumus

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  1. Gérald Larrouy-Maumus
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Editors and Affiliations

  1. Mass Spectrometry Resource Division of Endocrinology Metabolism, and Lipid Research Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA

    Fong-Fu Hsu

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Larrouy-Maumus, G. (2021). Shotgun Bacterial Lipid A Analysis Using Routine MALDI-TOF Mass Spectrometry. In: Hsu, FF. (eds) Mass Spectrometry-Based Lipidomics. Methods in Molecular Biology, vol 2306. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1410-5_18

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  • DOI: https://doi.org/10.1007/978-1-0716-1410-5_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1409-9

  • Online ISBN: 978-1-0716-1410-5

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