Abstract
The Bac-to-Bac® Baculovirus Expression System provides a rapid and efficient method to generate recombinant cryptochrome (CRY) proteins with chromophore flavin (FAD), which showed blue light response in vitro.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ahmad M, Cashmore AR (1993) HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor. Nature 366:162–166
Lin C, Ahmad M, Chan J et al (1995) CRY2, a second member of the arabidopsis cryptochrome gene family. Plant Physiol 110:1047
Cashmore AR (2003) Cryptochromes:enabling plants and animals to determine circadian time. Cell 114:537–543
Partch CL, Sancar A (2005) Cryptochromes and circadian photoreception in animals. Methods Enzymol 393:726–745
Guo H, Yang H, Mockler TC et al (1998) Regulation of flowering time by Arabidopsis photoreceptors. Science 279(5355):1360–1363
Somers DE, Devlin PF, Kay SA (1998) Phytochromes and cryptochromes in the entrainment of the Arabidopsis circadian clock. Science 282:1488–1490
Tsuchida-Mayama T, Sakai T, Hanada A et al (2010) Role of the phytochrome and cryptochrome signaling pathways in hypocotyl phototropism. Plant J 62(4):653–662
Lin C, Robertson DE, Ahmad M et al (1995) Association of flavin adenine dinucleotide with the Arabidopsis blue light receptor CRY1. Science 269:968–970
Malhotra K, Kim ST, Batschauer A et al (1995) Putative blue-light photoreceptors from Arabidopsis thaliana and Sinapis alba with a high degree of sequence homology to DNA photolyase contain the two photolyase cofactors but lack DNA repair activity. Biochemistry 34:6892–6899
Banerjee R, Schleicher E, Meier S et al (2007) The signaling state of Arabidopsis cryptochrome 2 contains flavin semiquinone. J Biol Chem 282:14916–14922
Bouly JP, Schleicher E, Dionisio-Sese M et al (2007) Cryptochrome blue light photoreceptors are activated through interconversion of flavin redox states. J Biol Chem 282:9383–9391
Bac-to-Bac® Baculovirus Expression System (2010) An efficient site-specific transposition system to generate baculovirus for high-level expression of recombinant proteins. Version F
Liu H, Yu X, Li K et al (2008) Photoexcited CRY2 interacts with CIB1 to regulate transcription and floral initiation in Arabidopsis. Science 322(5907):1535–1539
Li X, Wang QYXH et al (2011) Arabidopsis cryptochrome 2 (CRY2) functions by the photoactivation mechanism distinct from the tryptophan (trp) triad dependent photoreduction. Proc Natl Acad Sci U S A 108(51):20844–20849
Gao J, Wang X, Zhang M et al (2015) Trp triad-dependent rapid photoreduction is not required for the function of Arabidopsis CRY1. Proc Natl Acad Sci U S A 112:9135–9140
Liu YW, Li X, Ma D et al (2018) CIB1 and CO interact to mediate CRY2 regulation of flowering. EMBO Reports. https://doi.org/10.15252/embr.201845762
Acknowledgments
This work was supported by the National Natural Science Foundation of China (31730009, 31721001).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Li, X., Liu, Y., Liu, H. (2021). Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System. In: Yin, R., Li, L., Zuo, K. (eds) Plant Photomorphogenesis. Methods in Molecular Biology, vol 2297. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1370-2_16
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1370-2_16
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1369-6
Online ISBN: 978-1-0716-1370-2
eBook Packages: Springer Protocols