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Infant Rabbit Model for Studying Shiga Toxin-Producing Escherichia coli

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Shiga Toxin-Producing E. coli

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2291))

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Abstract

Animal models represent part of the arsenal available to researchers studying the pathophysiology of potentially deadly human pathogens such as Shiga toxin-producing Escherichia coli (STEC). The optimal model may differ depending on what aspects of pathogen biology, disease progression, or host response are under study. Here, we provide detailed protocols for the infant rabbit model of STEC, which largely reproduces the intestinal disease seen following natural oral infection, and share insights from studies examining O157 and non-O157 serotypes.

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Acknowledgment

The experimental expertize described in this chapter is the result of many years work by the author first in the laboratory of Professor Matthew Waldor (Brigham and Women’s Hospital / Harvard Medical School, Boston, USA) and more recently while based as an independent researcher at the University of Surrey. Work was supported in part by the European Union 7th Framework Programme grant entitled “Anticipating the global onset of novel epidemics” (project number 278976).

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Authors and Affiliations

  1. University of Surrey, Guildford, UK

    Jennifer M. Ritchie

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  1. Jennifer M. Ritchie
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Correspondence to Jennifer M. Ritchie .

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Editors and Affiliations

  1. University of East Anglia, Norwich Medical School, Norwich, UK

    Stephanie Schüller

  2. National Reference Laboratory for E. coli and Shigellae, National Institute of Public Health, Prague, Czech Republic

    Martina Bielaszewska

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Ritchie, J.M. (2021). Infant Rabbit Model for Studying Shiga Toxin-Producing Escherichia coli. In: Schüller, S., Bielaszewska, M. (eds) Shiga Toxin-Producing E. coli . Methods in Molecular Biology, vol 2291. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1339-9_18

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  • DOI: https://doi.org/10.1007/978-1-0716-1339-9_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1338-2

  • Online ISBN: 978-1-0716-1339-9

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