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In Vitro Anther Culture for Doubled Haploid Plant Production in Spelt Wheat

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Doubled Haploid Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2287))

Abstract

Doubled haploid (DH) plant production belongs to modern biotechnology methods of plant breeding. The main advantage of DH plant production methods is the development of genetically homozygous lines in one generation, whilst in conventional breeding programmes, the development of homozygous lines requires more generations. The present chapter describes an efficient protocol for DH plant production in spelt wheat genotypes using in vitro anther culture.

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Acknowledgements

This project was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences. The experiments were interlocked with scientific programmes (project code: OTKA-K_16-K119835; name of the project: Improvement of spelt wheat lines with low fermentable carbohydrate content (FODMAP) using modern and classical research methods), Thematic Excellence Programme 2019 (project code: TUDFO/51757/2019-ITM, supporter: National Research, Development and Innovation Office) and GINOP project (project number: GINOP-2.2.1-15-2016-00026). The authors thank the conscientious work of Ferenc Markó, Krisztina Kéri and Sándor Vajasdi-Nagy. Furthermore, the authors also thank László Láng (Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary) and Center for Plant Diversity (Tápiószele, gene bank of Hungary) for supplying the tested spelt wheat varieties (‘Franckenkorn’, ‘Mv Martongold’ and ‘Oberkulmer Rotkorn’) and gene bank germplasms (RCAT056296, RCAT058694, RCAT060960) for experiments, respectively.

Funding: This research was funded by the Hungarian Academy of Sciences, grant number ‘János Bolyai Research Scholarship’; National Research, Development and Innovation Office, grant number ‘OTKA-K_16-K119835’, ‘GINOP-2.2.1-15-2016-00026’ and ‘TUDFO/51757/2019-ITM’.

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Authors and Affiliations

  1. Department of Biotechnology, Cereal Research Non-profit Ltd., Szeged, Hungary

    Csaba Lantos & János Pauk

Authors
  1. Csaba Lantos
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  2. János Pauk
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Correspondence to János Pauk .

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Editors and Affiliations

  1. CPI, COMAV - Univ Politecnica Valencia, Valencia, Valencia, Spain

    Jose M. Segui-Simarro

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Lantos, C., Pauk, J. (2021). In Vitro Anther Culture for Doubled Haploid Plant Production in Spelt Wheat. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1315-3_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1315-3_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1314-6

  • Online ISBN: 978-1-0716-1315-3

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