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Identification of Follicular T-Cell Subsets in Murine and Human Tissues

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T-Helper Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2285))

Abstract

Follicular helper T (Tfh) cells play a key role in B cell activation and differentiation. Within recent years, distinct subsets of follicular T cells, including regulatory and cytotoxic T cells, have been identified. Apart from classical Tfh cells in secondary lymphoid organs, Tfh-like cells are found in chronically inflamed nonlymphoid tissues. Here, we provide protocols to identify different follicular T cell subsets in murine and human tissues by flow cytometry. This chapter also contains an immunization protocol for the induction of large numbers of Tfh cells in mice.

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Author information

Authors and Affiliations

  1. Chronic Immune Reactions, German Rheumatism Research Centre, A Leibniz Institute, Berlin, Germany

    Annette Lahmann, Laura Bauer & Andreas Hutloff

  2. Institute of Immunology, University Hospital Schleswig-Holstein, Kiel, Germany

    Laura Bauer & Andreas Hutloff

Authors
  1. Annette Lahmann
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  2. Laura Bauer
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  3. Andreas Hutloff
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Corresponding author

Correspondence to Andreas Hutloff .

Editor information

Editors and Affiliations

  1. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

    Francesco Annunziato

  2. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

    Laura Maggi

  3. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

    Alessio Mazzoni

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Lahmann, A., Bauer, L., Hutloff, A. (2021). Identification of Follicular T-Cell Subsets in Murine and Human Tissues. In: Annunziato, F., Maggi, L., Mazzoni, A. (eds) T-Helper Cells. Methods in Molecular Biology, vol 2285. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1311-5_6

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  • DOI: https://doi.org/10.1007/978-1-0716-1311-5_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1310-8

  • Online ISBN: 978-1-0716-1311-5

  • eBook Packages: Springer Protocols

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