Abstract
The CRISPR/Cas technology allows for genome editing in primary T cells. We herein describe the activation of primary murine CD4+ or CD8+ T cells, followed by electroporation with plasmid or ribonucleoproteins (RNP) for gene modification. Gene edited T cells can subsequently be transferred to host mice for in vivo studies or cultured in vitro for further characterization. This protocol enables sophisticated genetic analysis of T cells using commonly available virus-free reagents.
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Dölz, M., Marone, R., Jeker, L.T. (2021). Plasmid- or Ribonucleoprotein-Mediated CRISPR/Cas Gene Editing in Primary Murine T Cells. In: Annunziato, F., Maggi, L., Mazzoni, A. (eds) T-Helper Cells. Methods in Molecular Biology, vol 2285. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1311-5_20
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DOI: https://doi.org/10.1007/978-1-0716-1311-5_20
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