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Identification of Human Antigen-Specific CD4+ Cells with Peptide-MHC Multimer Technologies

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T-Helper Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2285))

Abstract

Peptide-major histocompatibility complex class II (pMHCII) multimers have emerged as a convenient and powerful tool for characterization of CD4 T cell immune responses in a large variety of human diseases. Peptide-MHCII multimers can rapidly identify peptide antigens recognized by CD4 T cells via high-throughput peptide screening procedures. The specificity and phenotype of antigen-specific CD4 T cells can be effectively visualized by pMHCII multimers from unmanipulated immune cell populations. Functional attributes of antigen-specific CD4 T cells can also be defined with the multimer technology in combination with immune functional assays such as intracellular cytokine staining (ICS).

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Acknowledgments

Supported by US National Institutes of Health NIDDK grant DP3DK106909.

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Authors and Affiliations

  1. The Benaroya Research Institute at Virginia Mason, Seattle, WA, USA

    I-Ting Chow & William W. Kwok

  2. The Department of Medicine, University of Washington, Seattle, WA, USA

    William W. Kwok

Authors
  1. I-Ting Chow
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  2. William W. Kwok
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Corresponding author

Correspondence to William W. Kwok .

Editor information

Editors and Affiliations

  1. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

    Francesco Annunziato

  2. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

    Laura Maggi

  3. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

    Alessio Mazzoni

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Chow, IT., Kwok, W.W. (2021). Identification of Human Antigen-Specific CD4+ Cells with Peptide-MHC Multimer Technologies. In: Annunziato, F., Maggi, L., Mazzoni, A. (eds) T-Helper Cells. Methods in Molecular Biology, vol 2285. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1311-5_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1311-5_13

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1310-8

  • Online ISBN: 978-1-0716-1311-5

  • eBook Packages: Springer Protocols

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