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Measuring Conjugated Linoleic Acid (CLA) Production by Bifidobacteria

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Bifidobacteria

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2278))

Abstract

The biological significance of conjugated fatty acids (CFAs) has been linked to positive health effects based on biomedical, in vitro, and clinical studies. Of note, conjugated linoleic acids (CLAs) are the most widely characterized fatty acids as geometric isomers cis-9,trans-11 and trans-10,cis-12 CLA occur naturally in ruminant fats, dairy products, and hydrogenated oils. Concerning CLAs, it is known that bacterial biohydrogenation, a process whereby ruminal bacteria or starter cultures of lactic acid bacteria have the ability to synthesize CLA by altering the chemical structure of essential fatty acids via enzymatic mechanisms, produces a multitude of isomers with desirable properties. Bifidobacterium species are classed as food grade microorganisms and some of these strains harness molecular determinants that are responsible for the bioconversion of free fatty acids to CLAs. However, molecular mechanisms have yet to be fully elucidated. Reports pertaining to CLAs have been attributed to suppressing tumor growth, delaying the onset of diabetes mellitus and reducing body fat in obese individuals. Given the increased attention for their bioactive properties, we describe in this chapter the qualitative and quantitative methods used to identify and quantify CLA isomers produced by bifidobacterial strains in supplemented broth media. These approaches enable rapid detection of potential CLA producing strains and accurate measurement of fatty acids in biological matrices.

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Author information

Authors and Affiliations

  1. Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland

    Grace Ahern & Catherine Stanton

  2. School of Microbiology, University College Cork, Cork, Ireland

    Grace Ahern

  3. APC Microbiome Ireland, University College Cork, Cork, Ireland

    Grace Ahern, R. Paul Ross & Catherine Stanton

  4. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, P. R. China

    Bo Yang

  5. School of Microbiology & APC Microbiome Ireland, University College Cork, Cork, Ireland

    Douwe van Sinderen

Authors
  1. Grace Ahern
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  2. Douwe van Sinderen
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  3. Bo Yang
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  4. R. Paul Ross
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  5. Catherine Stanton
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Corresponding author

Correspondence to Catherine Stanton .

Editor information

Editors and Affiliations

  1. Microbiome Ireland, School of Microbiology & APC, University College Cork, Cork, Ireland

    Douwe van Sinderen

  2. Laboratory of Probiogenomics, Department of Chemistry, Life Sciences & Environmental Sustainability, University of Parma, Parma, Italy

    Marco Ventura

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Ahern, G., van Sinderen, D., Yang, B., Ross, R.P., Stanton, C. (2021). Measuring Conjugated Linoleic Acid (CLA) Production by Bifidobacteria. In: van Sinderen, D., Ventura, M. (eds) Bifidobacteria. Methods in Molecular Biology, vol 2278. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1274-3_8

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  • DOI: https://doi.org/10.1007/978-1-0716-1274-3_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1273-6

  • Online ISBN: 978-1-0716-1274-3

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