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Investigating the Adipose Tissue Secretome: A Protocol to Generate High-Quality Samples Appropriate for Comprehensive Proteomic Profiling

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Proteomic Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2261))

Abstract

In this chapter we describe in detail how to prepare a sample containing the complete set of secretion products from primary adipocytes, which are suitable for comprehensive and sensitive secretome analysis. The underlying protocol should be seen as starting point guiding through critical steps of the complex workflow of preperation of secretomes. For diverse research questions and in the context of different sample types used, the protocol has to be carefully adjusted in order to approximate to the real secretome.

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Authors and Affiliations

  1. Institute for Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University Duesseldorf, Duesseldorf, Germany

    Sonja Hartwig, Jorg Kotzka & Stefan Lehr

  2. German Center for Diabetes Research, München-Neuherberg, Germany

    Sonja Hartwig, Jorg Kotzka & Stefan Lehr

Authors
  1. Sonja Hartwig
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  2. Jorg Kotzka
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  3. Stefan Lehr
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Corresponding author

Correspondence to Stefan Lehr .

Editor information

Editors and Affiliations

  1. Bio-Rad Laboratories GmbH, Feldkirchen, Germany

    Anton Posch

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Hartwig, S., Kotzka, J., Lehr, S. (2021). Investigating the Adipose Tissue Secretome: A Protocol to Generate High-Quality Samples Appropriate for Comprehensive Proteomic Profiling. In: Posch, A. (eds) Proteomic Profiling. Methods in Molecular Biology, vol 2261. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1186-9_26

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  • DOI: https://doi.org/10.1007/978-1-0716-1186-9_26

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1185-2

  • Online ISBN: 978-1-0716-1186-9

  • eBook Packages: Springer Protocols

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