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Induced Dimerization Tools to Deplete Specific Phosphatidylinositol Phosphates

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Phosphoinositides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2251))

Abstract

Chemical dimerization systems have been used to drive acute depletion of polyphosphoinsitides (PPIns). They do so by inducing subcellular localization of enzymes that catabolize PPIns. By using this approach, all seven PPIns can be depleted in living cells and in real time. The rapid permeation of dimerizer agents and the specific expression of recruiter proteins confer great spatial and temporal resolution with minimal cell perturbation. In this chapter, we provide detailed instructions to monitor and induce depletion of PPIns in live cells.

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Acknowledgments

This work was supported by National Institutes of Health grant 1R35GM119412-01 (to G.R.V. Hammond).

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Authors and Affiliations

  1. Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Jonathan Pacheco, Rachel C. Wills & Gerald R. V. Hammond

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  1. Jonathan Pacheco
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  2. Rachel C. Wills
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  3. Gerald R. V. Hammond
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Correspondence to Gerald R. V. Hammond .

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Editors and Affiliations

  1. Department of Chemistry and Biology and the Graduate Program in Molecular Science, Ryerson University, Toronto, ON, Canada

    Roberto J. Botelho

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Pacheco, J., Wills, R.C., Hammond, G.R.V. (2021). Induced Dimerization Tools to Deplete Specific Phosphatidylinositol Phosphates. In: Botelho, R.J. (eds) Phosphoinositides. Methods in Molecular Biology, vol 2251. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1142-5_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1142-5_7

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  • Publisher Name: Humana, New York, NY

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