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Production of Multi-Subtype Influenza Virus-Like Particles by Molecular Fusion with BAFF or APRIL for Vaccine Development

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The TNF Superfamily

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2248))

Abstract

Virus-like particle (VLP) technology is an alternative platform for developing vaccines to combat seasonal and pandemic influenza. Influenza VLPs are non-infectious nanoparticles that can elicit effective vaccine immunogenicity in hosts. B-cell-activating factor (BAFF, or BLyS) and a proliferation-inducing ligand (APRIL) are members of the tumor necrosis factor (TNF) superfamily of cytokines. Both BAFF and APRIL are homotrimers that interact with homotrimeric receptors. Here, we report a method of the production of influenza VLPs by molecular incorporation with BAFF or APRIL homotrimers to interact with their receptors. We engineered the VLPs by direct fusion of BAFF or APRIL to the transmembrane anchored domain of the hemagglutinin (HA) gene. We also describe procedures for the production of BAFF-VLPs containing H5H7 and H1H5H7 for multi-subtype vaccine development.

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Acknowledgments

We thank the International Institute of Macromolecular Analysis and Nanomedicine Innovation, National Cheng Kung University, Taiwan, for TEM analysis. This work was supported by the Ministry of Science and Technology, Taiwan (MOST108-2321-B-007-001, MOST108-2321-B-002-006).

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Authors and Affiliations

  1. Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan

    Ting-Hsuan Chen, Jo-Yu Hong & Suh-Chin Wu

  2. National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan

    Chia-Chyi Liu

  3. Hsinchu MacKay Memorial Hospital, Hsinchu, Taiwan

    Chung-Chu Chen

  4. Genomics Research Center, Academia Sinica, Taipei, Taiwan

    Jia-Tsrong Jan

  5. Department of Medical Science, National Tsing Hua University, Hsinchu, Taiwan

    Suh-Chin Wu

Authors
  1. Ting-Hsuan Chen
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  2. Jo-Yu Hong
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  3. Chia-Chyi Liu
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  4. Chung-Chu Chen
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  5. Jia-Tsrong Jan
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  6. Suh-Chin Wu
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Corresponding author

Correspondence to Suh-Chin Wu .

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Editors and Affiliations

  1. INSERM, Sorbonne Université, Paris, France

    Jagadeesh Bayry

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Chen, TH., Hong, JY., Liu, CC., Chen, CC., Jan, JT., Wu, SC. (2021). Production of Multi-Subtype Influenza Virus-Like Particles by Molecular Fusion with BAFF or APRIL for Vaccine Development. In: Bayry, J. (eds) The TNF Superfamily. Methods in Molecular Biology, vol 2248. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1130-2_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1130-2_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1129-6

  • Online ISBN: 978-1-0716-1130-2

  • eBook Packages: Springer Protocols

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