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Laser Capture Microdissection of Mouse Growth Plate Cartilage

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Chondrocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2245))

Abstract

The ability to identify, isolate, and study pure populations of cells is critical for understanding normal physiology in organs and tissues, which involves spatial regulation of signaling pathways and interactions between cells with different functions, expression profiles, and lineages. Here, we focus on assessing the growth plate cartilage, composed of multiple functionally and histologically distinct zones, to investigate temporally and spatially dependent gene expression differences. In this chapter, we describe the method of laser capture microdissection to isolate chondrocytes from different zones of differentiation in the mouse growth plate cartilage for RNA isolation, and subsequent downstream applications, such as RNA-sequencing and quantitative real-time PCR. We also provide an assessment of different factors contributing to the integrity of the isolated RNA, such as staining methods and procedures in RNA isolation.

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Author information

Authors and Affiliations

  1. Section on Growth and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA

    Bijal Kikani & Julian C. Lui

Authors
  1. Bijal Kikani
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  2. Julian C. Lui
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Corresponding author

Correspondence to Julian C. Lui .

Editor information

Editors and Affiliations

  1. Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, OH, USA

    Tariq M. Haqqi

  2. Department of Surgery, The Children's Hospital of Philadelphia, Philadelphia, PA, USA

    Véronique Lefebvre

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Kikani, B., Lui, J.C. (2021). Laser Capture Microdissection of Mouse Growth Plate Cartilage. In: Haqqi, T.M., Lefebvre, V. (eds) Chondrocytes. Methods in Molecular Biology, vol 2245. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1119-7_8

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  • DOI: https://doi.org/10.1007/978-1-0716-1119-7_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1118-0

  • Online ISBN: 978-1-0716-1119-7

  • eBook Packages: Springer Protocols

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