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Using Primary Human Cells to Analyze Human Cytomegalovirus Biology

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Human Cytomegaloviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2244))

Abstract

The extensive tropism of human cytomegalovirus (HCMV) results in the productive infection of multiple cell types within the human host. However, infection of other cell types, such as undifferentiated cells of the myeloid lineage, give rise to nonpermissive infections. This aspect has been used experimentally to model latent infection, which is known to be established in the pluripotent CD34+ hematopoietic progenitor cell population resident in the bone marrow in vivo. The absence of a tractable animal model for studies of HCMV has resulted in a number of laboratories employing experimental infection of cells in vitro to simulate both HCMV lytic and latent infection. Herein, we will focus on the techniques used in our laboratory for the isolation and use of primary cells to study aspects of HCMV latency, reactivation, and lytic infection.

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Authors and Affiliations

  1. Department of Medicine, Addenbrookes Hospital, University of Cambridge, Cambridge, UK

    Emma Poole, Ian Groves, Sarah Jackson, Mark Wills & John Sinclair

Authors
  1. Emma Poole
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  2. Ian Groves
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  3. Sarah Jackson
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  4. Mark Wills
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  5. John Sinclair
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Corresponding author

Correspondence to Emma Poole .

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Editors and Affiliations

  1. Department of Microbiology and Immunology, Louisiana State University Health Sciences Center—Shreveport, Shreveport, LA, USA

    Andrew D. Yurochko

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Poole, E., Groves, I., Jackson, S., Wills, M., Sinclair, J. (2021). Using Primary Human Cells to Analyze Human Cytomegalovirus Biology. In: Yurochko, A.D. (eds) Human Cytomegaloviruses. Methods in Molecular Biology, vol 2244. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1111-1_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1111-1_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1110-4

  • Online ISBN: 978-1-0716-1111-1

  • eBook Packages: Springer Protocols

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