Abstract
Since its introduction in 1971, the enzyme-linked immunosorbent assay (ELISA) has revolutionized medicine by enabling detection of both antigens and antibodies in a variety of samples. We describe here a customized sandwich ELISA developed for the detection of Human Cytomegalovirus interleukin-10 (cmvIL-10). CmvIL-10 is a virally encoded cytokine and ortholog of human interleukin 10 (hIL-10). While cmvIL-10 and hIL-10 are similar in structure and function, overall amino acid sequence identity is only 27%, resulting in antigenically distinct proteins. The cmvIL-10 ELISA is specific and does not detect hIL-10. The assay is sensitive enough to detect cmvIL-10 in both culture supernatants and patient serum. The ability to quantify cmvIL-10 levels during HCMV infection could provide valuable information about immune evasion strategies and viral control of host signaling pathways.
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Acknowledgements
This work was supported by grants from the National Institute for Allergy and Infectious Disease of the National Institutes of Health (AI111232) and the Avon Foundation for Women (02-2014-052) to J.V.S.
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Young, V.P., Mariano, M.C., Faure, L., Spencer, J.V. (2021). Detection of Cytomegalovirus Interleukin 10 (cmvIL-10) by Enzyme-Linked Immunosorbent Assay (ELISA). In: Yurochko, A.D. (eds) Human Cytomegaloviruses. Methods in Molecular Biology, vol 2244. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1111-1_15
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DOI: https://doi.org/10.1007/978-1-0716-1111-1_15
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