Abstract
Radiolabeled leukocyte scans are used in nuclear medicine to detect sites of infection and inflammation. We have previously demonstrated the use of clinical grade immunomagnetic beads to isolate autologous eosinophils and image their distribution in healthy volunteers. Here we describe the use of radiolabeled eosinophils coupled to single-photon emission computed tomography (SPECT) to quantify eosinophil uptake in the lungs of healthy volunteers, patients with asthma, and patients with focal eosinophilic inflammation.
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Acknowledgments
This work was supported by Asthma-UK [08/11], the Medical Research Council [grant number MR/J00345X/1], the Wellcome Trust [grant number 098351/Z/12/Z], and Cambridge NIHR Biomedical Research Centre.
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Farahi, N., Gillett, D., Loutsios, C., Peters, A.M., Summers, C., Chilvers, E.R. (2021). Measurement of Eosinophil Kinetics In Vivo. In: Walsh, G.M. (eds) Eosinophils. Methods in Molecular Biology, vol 2241. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1095-4_15
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DOI: https://doi.org/10.1007/978-1-0716-1095-4_15
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