Abstract
The biological functions of a cell may change in response to exposure to toxic agents. Toxicogenomics employs the recent developments in genomics, transcriptomics, and proteomics to study how a chemical impacts gene/protein expression and cell functions. We describe a method for transcriptomic analysis by RNA sequencing based on Illumina HiSeq, NextSeq, or NovaSeq Systems followed by real-time qPCR validation. We also depict a method for proteomic analysis by “one-dimensional sodium dodecyl sulfate—polyacrylamide gel electrophoresis” (1D SDS-PAGE) and a sample preparation procedure for “liquid chromatography in tandem with mass spectrometry” (LC-MS/MS), and we present some generic points to consider during LC-MS/MS.
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Dinamarco, T.M., de Gouvêa, P.F., de Souza Santos, E., Sousa, L.O. (2021). Toxicogenomic Analysis. In: Palmeira, C.M.M., de Oliveira, D.P., Dorta, D.J. (eds) Toxicity Assessment. Methods in Molecular Biology, vol 2240. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1091-6_12
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DOI: https://doi.org/10.1007/978-1-0716-1091-6_12
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-1091-6
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