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Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance

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Nuclear Reprogramming

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2239))

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Abstract

Mouse embryonic fibroblasts (MEFs) can be used in co-culture to support generation of induced pluripotent stem cells (iPSCs) and the normal growth and proliferation of human pluripotent stem cells (hPSCs). Here, we describe the necessary steps to derive, expand, harvest, inactivate, plate, and use MEFs as feeders for iPSC generation and maintenance.

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Authors and Affiliations

  1. WiCell Research Institute, Madison, WI, USA

    Jennifer L. Brehm, Molly M. Miles, Katie S. Remondini & Tenneille E. Ludwig

Authors
  1. Jennifer L. Brehm
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  2. Molly M. Miles
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  3. Katie S. Remondini
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  4. Tenneille E. Ludwig
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Corresponding author

Correspondence to Tenneille E. Ludwig .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA

    Kejin Hu

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Brehm, J.L., Miles, M.M., Remondini, K.S., Ludwig, T.E. (2021). Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance. In: Hu, K. (eds) Nuclear Reprogramming. Methods in Molecular Biology, vol 2239. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1084-8_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1084-8_14

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1083-1

  • Online ISBN: 978-1-0716-1084-8

  • eBook Packages: Springer Protocols

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