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Purification of Trichoderma Producing Hydrolytic Enzyme: β-1,3 Glucanase

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Plant-Microbe Interactions

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Abstract

Hydrolytic enzyme β-1,3 glucanase cleaves the glycosidic bonds adjacent to glucose residues in β-1,3 glucan. This enzyme is inhibiting the growth of the fungi. In the present protocol, the purification of Trichoderma producing β 1,3 glucanase is narrated.

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References

  1. Bara MTF, Lima AL, Ulhoa CJ (2003) Purification and characterization of an exo- β 1,3-glucanase produced by Trichoderma asperellum. FEMS Microbiol Lett 219:81–85

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  2. Ramada MHS, Steindorff AS, Bloch C Jr, Ulhoa CJ (2016) Secretome analysis of the mycoparasitic fungus Trichoderma harzianum ALL 42 cultivated in different media supplemented with Fusarium solani cell wall or glucose. Proteomics 16:477–490

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Senthilkumar, M., Amaresan, N., Sankaranarayanan, A. (2021). Purification of Trichoderma Producing Hydrolytic Enzyme: β-1,3 Glucanase. In: Plant-Microbe Interactions. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1080-0_62

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  • DOI: https://doi.org/10.1007/978-1-0716-1080-0_62

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1079-4

  • Online ISBN: 978-1-0716-1080-0

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