Abstract
Purification is an important step in enzyme production which helped in removing the impurities from the enzyme. The maximum activity is exhibited after purification step. Ion exchange chromatographic technique is widely used to chromatographic technique for the separation and purification of proteins, biomolecules, and nucleic acids. This technique is functioning based on the isoelectric point of an enzyme.
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Reference
Senol M, Nadaroglu H, Dikbas N, Kotan R (2014) Purification of chitinase enzymes from Bacillus subtilis bacteria TV-125, investigation of kinetic properties and antifungal activity against Fusarium culmorum. Ann Clin Microbiol Antimicrob 13:351–357
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Senthilkumar, M., Amaresan, N., Sankaranarayanan, A. (2021). Partial Purification of Bacterial Chitinase Enzyme by Ion Exchange Chromatography. In: Plant-Microbe Interactions. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1080-0_59
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DOI: https://doi.org/10.1007/978-1-0716-1080-0_59
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1079-4
Online ISBN: 978-1-0716-1080-0
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