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One-Step Antibody Arrays

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Antibody Arrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2237))

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Abstract

The sandwich-based immunoassay is renowned for its specificity and sensitivity for protein detection, where the antigen is “sandwiched” by a pair of antigen epitope-specific antibodies. The capture antibody-antigen-detection antibody complex will be formed if all the components are present in a proper reaction system. A one-step rapid sandwich-based antibody array can be developed through fixing the capture antibody on a glass slide with a fluorescence-labelled detection antibody. In this chapter, we describe the process of a one-step mouse immunoglobulin isotyping array for use in hybridoma culture supernatants.

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References

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Author information

Authors and Affiliations

  1. RayBiotech Life, Peachtree Corners, GA, USA

    Ying Qing Mao

  2. Department of Laboratory Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong, China

    Lin-Hai Li

Authors
  1. Ying Qing Mao
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  2. Lin-Hai Li
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Corresponding author

Correspondence to Ying Qing Mao .

Editor information

Editors and Affiliations

  1. RayBiotech, Peachtree Corners, GA, USA

    Kelly C. Whittaker

  2. RayBiotech Life, Peachtree Corners, GA, USA

    Ruo-Pan Huang

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Cite this protocol

Mao, Y.Q., Li, LH. (2021). One-Step Antibody Arrays. In: Whittaker, K.C., Huang, RP. (eds) Antibody Arrays. Methods in Molecular Biology, vol 2237. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1064-0_3

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  • DOI: https://doi.org/10.1007/978-1-0716-1064-0_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1063-3

  • Online ISBN: 978-1-0716-1064-0

  • eBook Packages: Springer Protocols

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