Abstract
Flow cytometry and magnetic bead technology enable the separation of cell populations with the highest degree of purity. Here, we describe protocols to sort bovine neutrophils from blood, the labeling and sorting, including gating strategies. We also provide advice to preserve neutrophil viability and detail a protocol to measure phagocytosis and oxidative species production.
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Acknowledgments
Aude Remot was supported by a grant from the Agence Nationale de la Recherche under the Carnot Program France Future Elevage. Marion Rambault’s Ph.D. thesis was supported by a grant from the Institut de l’Élevage (Idele) Company (CIFRE N° 2019/0776). Rachana Borkute’s Ph.D. thesis was supported by FLI Intramural funds. We warmly thank Dr. Pascal Rainard for critical reading of the manuscript.
Eric Briant and his team (UE-PAO, INRAE, Nouzilly) and Bärbel Hammerschmidt (FLI, Greifswald- Insel Riems, Germany) are gratefully acknowledged for bovine blood sampling.
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Rambault, M. et al. (2021). Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting. In: Brandau, S., Dorhoi, A. (eds) Myeloid-Derived Suppressor Cells. Methods in Molecular Biology, vol 2236. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1060-2_16
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DOI: https://doi.org/10.1007/978-1-0716-1060-2_16
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Online ISBN: 978-1-0716-1060-2
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