Abstract
Pericytes are found in all vascularized organs and are defined anatomically as perivascular cells that closely surround endothelial cells in capillaries and microvessels and are embedded within the same basement membrane. They have been shown to have diverse physiological and pathological functions including regulation of blood pressure, and tissue regeneration and scarring. Fundamental to understanding the role these cells play in these diverse processes is the ability to accurately identify and localize them in vivo. To do this, we have developed multicolor immunohistochemistry protocols described in this chapter.
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Acknowledgments
The authors thank and acknowledge their funders, the European Haematology Association Non-clinical Advanced Fellowship, the University of Edinburgh Chancellor’s Fellowship, and the Edinburgh BHF Centre of Research Excellence to MC. CCW is funded by a Chief Scientist Office Clinical Academic Training Fellowship (CAF/11/13), a research grant from the William Rooney Plastic Surgery and Burns Research Trust, and a Surgical Research Fellowship from the Royal College of Surgeons of England.
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West, C.C., Khan, N.S., Crisan, M. (2021). Characterization of Human Pericyte Phenotype by Immunohistochemistry. In: Péault, B.M. (eds) Pericytes. Methods in Molecular Biology, vol 2235. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1056-5_4
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DOI: https://doi.org/10.1007/978-1-0716-1056-5_4
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1055-8
Online ISBN: 978-1-0716-1056-5
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