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Establishing Orthotopic Xenograft Glioblastoma Models for Use in Preclinical Development

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Nanotherapy for Brain Tumor Drug Delivery

Part of the book series: Neuromethods ((NM,volume 163))

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Abstract

Glioblastoma multiforme is the most common, aggressive and lethal type of brain tumor, characterized by an aggressive, heterogenic and highly angiogenic behavior. Establishing mice models that mimic the etiology, biology and histopathology of human glioblastomas is of extreme importance, as they are a crucial tool to understand the tumor initiation, formation, angiogenesis, progression and metastasis. Orthotopic xenograft mice models remain in the frontline of neuro-oncology as an experimental system to identify novel therapeutic targets and to determine the efficacy of different therapeutic agents and/or nanosystems. The present chapter describes a protocol for establishing brain tumor xenografts in mice following a single injection of glioblastoma cells.

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Acknowledgments

João Basso acknowledges the PhD research grant SFRH/BD/149138/2019 assigned by Fundação para a Ciência e a Tecnologia (FCT), the Portuguese Agency for Scientific Research. The authors also acknowledge FCT for the financial support through the Research Projects no. 016648 (Ref. POCI-01-0145-FEDER- 016648), Pest UID/NEU/04539/2013, COMPETE (Ref. POCI-01-0145-FEDER-007440), and CENTRO-01-0145-FEDER-030752 and the Coimbra Chemistry Centre, supported by FCT, through the Project UID/QUI/00313/2020.

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Authors and Affiliations

  1. Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, Coimbra, Portugal

    João Basso, Ana Fortuna & Carla Vitorino

  2. Centre for Neurosciences and Cell Biology (CNC), University of Coimbra, Faculty of Medicine, Coimbra, Portugal

    João Basso, Ana Fortuna & Carla Vitorino

  3. Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT), Edifício do ICNAS, Polo 3, Azinhaga de Santa Comba, Coimbra, Portugal

    José Sereno, Ana Fortuna & Miguel Castelo-Branco

  4. Institute of Nuclear Science Applied to Health (ICNAS), University of Coimbra, Polo 3, Azinhaga de Santa Comba, Coimbra, Portugal

    José Sereno & Miguel Castelo-Branco

  5. Coimbra Chemistry Centre, Department of Chemistry, University of Coimbra, Coimbra, Portugal

    Carla Vitorino

Authors
  1. João Basso
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  2. José Sereno
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  3. Ana Fortuna
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  4. Miguel Castelo-Branco
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  5. Carla Vitorino
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Corresponding author

Correspondence to Carla Vitorino .

Editor information

Editors and Affiliations

  1. CONRAD / Eastern Virginia Medical School, Arlington, VA, USA

    Vivek Agrahari

  2. Department of Neurosurgery and Pharmacology, University of Maryland School of Medicine, Baltimore, MD, USA

    Anthony Kim

  3. Department of Pharmaceutical Sciences, College of Pharmacy, University of Oklahoma Health Science Center, Oklahoma City, OK, USA

    Vibhuti Agrahari

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Basso, J., Sereno, J., Fortuna, A., Castelo-Branco, M., Vitorino, C. (2021). Establishing Orthotopic Xenograft Glioblastoma Models for Use in Preclinical Development. In: Agrahari, V., Kim, A., Agrahari, V. (eds) Nanotherapy for Brain Tumor Drug Delivery. Neuromethods, vol 163. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1052-7_12

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  • DOI: https://doi.org/10.1007/978-1-0716-1052-7_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1051-0

  • Online ISBN: 978-1-0716-1052-7

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